The former Millwall manager, who correctly tipped the Millers to be beaten 1-0 at Reading on Tuesday, thinks the Bees will win 2-1 at New York Stadium.
Holloway previously expected Rotherham to pull away from danger while Neil Redfearn was in charge but now says they will go down, believing their "bubble has burst".
He said in his Sky Sports prediction column: "It's going to be a dismal game this one, but Brentford have the edge. His name is Alan Judge. What a lovely footballer he is. He's turning plenty of heads with his performances and can win a game on his own.
"This will be Neil Warnock's toughest job
keeping Rotherham up. It looks as though their bubble has burst after three
years of success.
"I think I'd rather be
watching Soccer Saturday than be at this game. There are a
million reasons why I'll be watching and hoping for a 2-1 Brentford win as this
is the second Championship game in the Super 6 jackpot."
It is the 17th time from 19 predictions that Holloway has backed a Millers defeat, though most of them have hardly been the bravest of calls.
He has got 13 results correct and five scores correct, including the last two games.
